Willie Nelson made a guest appearance during The Muppets season finale on Tuesday night (March 1) to perform one of his classic songs, "On the Road Again."

On the show, Nelson performs the tune, released in 1980, for Miss Piggy while she is in the hospital. A crew of the Muppets -- together known as Electric Mayhem -- join the country icon for the song, playing additional instruments and adding harmonies as Miss Piggy, Kermit and some doctors and fellow patients look on. Press play on the video above to watch the smile-inducing performance.

Nelson teased his appearance on The Muppets at the end of January, sharing a photo of himself and a few of the Muppets in a bar setting. Readers can see the photo below.

Nelson's appearance on The Muppets season finale is not the first time his path has crossed with the beloved characters: In 1989, the Muppets sang "On the Road Again" during an episode of The Jim Henson Hour, while Nelson himself recorded "Rainbow Connection" -- which Kermit sings in 1979's The Muppet Movie and which became a Top 30 hit as sung by the frog -- for his 2001 album of the same name.

See Willie Nelson + More Country Stars in Their Early Days

How Well Do You REALLY Know Willie Nelson?

More From KLAW-FM